Single cell RNA sequencing illustrates individual cells with thousands of gene expression, which allows dissection of heterogeneous cell populations in complex tissues. Thus it is a powerful technique to study cellular landscape in normal tissues providing high-resolution criteria for health and disease. Cancer is a disease of genomic instability and tumor heterogeneity has been suspected as a major mechanism inducing treatment resistance of molecular targeted therapies. Using single cell RNA sequencing, it is possible to visualize heterogeneous gene expression patterns in each tumor cell which determine the susceptibility or resistance to molecular targeted drugs. Thus, drug targets or drug combinations can be suggested based on the information from the single cell data. The power of single cell RNA sequencing is also appreciated in the study of tumor microenvironment. Simultaneous characterization of diverse stromal and immune cells enables comparative analyses of responders and non-responders to immune therapies. This will improve the process of patient stratification and also accelerate selecting or developing new options for immune targeted therapies.
BS, Seoul National University 1993
PhD., Immunology and Microbial Pathogenesis, Northwestern University 1999
Chief Researcher, Samsung Genome Institute, Samsung Medical Center 2013~2020
Trained in Immunology, Hae-Ock Lee has been studying cancer using patient samples and mouse tumor models. She is one of the pioneers who adopted single cell RNA sequencing to understand tumor heterogeneity and diverse cellular interactions in the tumor microenvironment. Currently, she is working on the translation of the findings in the single cell RNA sequencing data into strategic development of cancer therapeutics.
Recent publications
Kim, N., et al., Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma. Nat Commun, 2020. 11(1): p. 2285.
Lee, H.O., et al., Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer. Nat Genet, 2020. 52(6): p. 594-603
Lee, H.W., et al., Single-cell RNA sequencing reveals the tumor microenvironment and facilitates strategic choices to circumvent treatment failure in a chemorefractory bladder cancer patient. Genome Med, 2020. 12(1): p. 47.
Ryu, D., et al., Alterations in the Transcriptional Programs of Myeloma Cells and the Microenvironment during Extramedullary Progression Affect Proliferation and Immune Evasion. Clin Cancer Res, 2020. 26(4): p. 935-944.